General Cell Based Assays
Eurofins Discoverx PathHunter eXpress IFNRL1/IL10RB Dimerization Assay, 2pl Kit. (93-0993E3CP5M)
The PathHunter IFNRL1/IL10RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-29) induced dimerization of IFNRL1/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IFNRL1 target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IFNRL1/IL10RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human IL-2 (IL-2R (IL2RA/IL2RB/IL2RG)) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-2 (IL-2R (IL2RA/IL2RB/IL2RG)) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL2RB/IL2RG/IL2RA. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human EDNRB Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human EDNRB Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure EDNRB (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human HTR2C (5-HT2C) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human HTR2C(VNV) (5-HT2C) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure HTR2C(VNV) (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human p53 (TP53) Nuclear Translocation Cell-Based Assay Kit (U2OS)
The PathHunter Human p53 (TP53) Stable Cell Line Nuclear Translocation Assay (U2OS) contains a stable clonal cell line used to measure p53 DNA damage pathway activation via nuclear translocation of p53. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Revvity Health Sciences Inc HTRF Human AQ STING Binding Kit, 100,000 Assay Points
HTRF Human AQ STING Binding Kit, 100,000 Assay Points
Eurofins Discoverx PathHunter CXCR5 Bioassay Kit, 2-Plate. (93-0514Y2-00063)
The PathHunter CXCR5 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CXCL13) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR5 and EA-tagged β-Arrestin-2. Activation of CXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Revvity Health Sciences Inc HTRF Human α-SMA Detection Kit, 100,000 Assay Points
HTRF Human α-SMA Detection Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Mouse STING WT Binding Kit, 100,000 Assay Points
HTRF Mouse STING WT Binding Kit, 100,000 Assay Points
Eurofins Discoverx PathHunter eXpress SSTR2 Activated GPCR Internalization Assay, 2pl Kit. (93-0676E2CP0M)
The PathHunter SSTR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of SSTR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TACR2 Activated GPCR Internalization Assay, 10pl Kit. (93-0677E3CP0L)
The PathHunter TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of TACR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR173 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0380E2ACP1L)
The PathHunter GPR173 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR173 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR173 and EA-tagged β-Arrestin-2. Activation of GPR173 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF pAb Anti-Mouse IgG Eu-Conjugate, 5,000 Assay Points
HTRF pAb Anti-Mouse IgG Eu-Conjugate, 5,000 Assay Points
Revvity Health Sciences Inc HTRF mAb Anti-FLAG Eu-Conjugate, 5,000 Assay Points
HTRF mAb Anti-FLAG Eu-Conjugate, 5,000 Assay Points
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0076E2CP2M)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.